Anti-Midkine Antibody (A12574)

Name: Anti-Midkine Antibody
Brand: Antibodies.com
SKU: A12574
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Midkine for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Midkine Antibody

Description

Rabbit polyclonal antibody to Midkine.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-129 of human MDK (NP_002382.1).

Sequence

SECAEWAWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFENWGACDGGTGTKVRQGTLKKARYNAQCQETIRVTKPCTPK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Amphiregulin-associated protein, ARAP, MDK, Midgestation and kidney protein, MK, MK1, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Midkine Antibody (A12574)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-Midkine Antibody (A12574) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunofluorescence - Anti-Midkine Antibody (A12574)

Immunofluorescence analysis of HeLa cells using Anti-Midkine Antibody (A12574) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Midkine Antibody (A12574)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Midkine Antibody (A12574) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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