Anti-MHC Class II Antibody [PF8J-9B] (A281557)

Anti-MHC Class II Antibody [PF8J-9B]

Mouse monoclonal [PF8J-9B] antibody to MHC Class II.

This antibody recognises an epitope within the ~30 kDa beta chain region of cat MHC class II molecule, expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.The complex retrovirus FIV (feline immunodeficiency virus), is responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency virus (HIV). The intensity of MHC Class II expression on cat lymphocyte membranes vary in association with feline retrovirus infections.

WB, ELISA, IF, Flow Cytometry

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Feline

Mouse

Monoclonal

PF8J-9B

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Approximately 29-31 kDa on fractionated cat lymphocyte cell lysates under non-reducing conditions.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Cat MHC class II antibody, clone PF8J-9B recognizes an epitope within the ~30 kDa beta chain region of cat MHC class II molecule, expressed by antigen presenting cells, B cells, monocytes and activated T lymphocytes. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.The complex retrovirus FIV (feline immunodeficiency virus), is responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency virus (HIV). The intensity of MHC Class II expression on cat lymphocyte membranes vary in association with feline retrovirus infections (Ohno et al. 1992).