Anti-MHC Class II Antibody [M5/114] (FITC) (A85588)

Rat monoclonal [M5/114] antibody to MHC Class II (FITC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-MHC Class II Antibody [M5/114] (FITC)
Description
Rat monoclonal [M5/114] antibody to MHC Class II (FITC).
Specificity
This antibody recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 2-3 µg/ml.
Reactivity
Mouse
Immunogen
Activated C57BL/6 mouse spleen cells.
Host
Rat
Clonality
Monoclonal
Clone ID
M5/114
Isotype
IgG2b
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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