Western Blot - Anti-Metallothionein Antibody (A305192)
Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Anti-Metallothionein Antibody (A305192) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 µg. Block: 1% milk, 2% BSA. The secondary antibody used was mouse Anti-Rabbit IgG: AP at 1:1,000 for 60 minutes at room temperature. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).
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