Anti-Mesothelin Antibody [DM71] - BSA and Azide free
Description
Recombinant rabbit monoclonal [DM71] antibody to Mesothelin.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM71
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Expi 293 cell line was transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392)
ELISA plates were pre-coated with Recombinant Human Mesothelin Protein (Fc Chimera 6xHis Tag) (A318377) at 2 µg/ml (100 µl/well) which can bind Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) in a linear range of 1-100 ng/ml.
Flow cytometry data of serially titrated Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-MSLN Monoclonal Antibody clones by titration of different concentrations onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392). (A) Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) does not bind to 293T cells that do not express Mesothelin. (B) A clear peak shift of Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) to Mesothelin. Antibodies were incubated at 2 µg/ml.
Immunohistochemistry analysis of Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392). A. Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) at 10µg/ml staining MSLN in Hela cells. B. Anti-Mesothelin Antibody [DM71] - BSA and Azide free (A317392) at 10µg/ml staining MSLN in human tonsil tissue.
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