Recombinant humanized monoclonal antibody to IL-5.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 146.24 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Mepolizumab Biosimilar - Anti-IL-5 Antibody - Low endotoxin, Azide free (A323575) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Mepolizumab Biosimilar - Anti-IL-5 Antibody - Low endotoxin, Azide free (A323575) confirms that the purity of this antibody is 98.47%.
Mepolizumab Biosimilar - Anti-IL-5 Antibody - Low endotoxin, Azide free (A323575) induced FACS blocking was evaluated using Human IL-5Rß-FL/HIL-5Ra CHOS. The IC50 was approximately 2.844 µg/ml.
Mepolizumab Biosimilar - Anti-IL-5 Antibody - Low endotoxin, Azide free (A323575) induced proliferation inhibition activity was evaluated using human IL-5Ra TF-1. The EC50 was approximately 0.3282 µg/ml.
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