Recombinant Anti-Melanoma gp100 Antibody [PMEL/1825R] - BSA and Azide free (A253156)

Anti-Melanoma gp100 Antibody [PMEL/1825R] - BSA and Azide free

Recombinant rabbit monoclonal [PMEL/1825R] antibody to Melanoma gp100.

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

WB, IHC-P

WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml

Human

This antibody does not cross react with Rat or Canine.

Recombinant full-length human gp100 protein.

Rabbit

Monoclonal

PMEL/1825R

Protein A/G chromatography.

1 mg/ml

26-100 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Melanoma gp100 Antibody [PMEL/1825R] (A249976).