Anti-Melanoma gp100 Antibody [HMB45 + PMEL/783] (A249972)

Anti-Melanoma gp100 Antibody [HMB45 + PMEL/783]

Mouse monoclonal [HMB45 + PMEL/783] antibody to Melanoma gp100.

The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

WB, IHC-P

WB: 2-4 µg/ml, IHC-P: 1-2 µg/ml

Human

Clone HMB45: Extract of pigmented melanoma metastases from lymph nodes. Clone PMEL/783: Recombinant human SILV protein.

Mouse

Oligoclonal

HMB45 + PMEL/783

Protein A/G chromatography.

200 µg/ml

26-100 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.