Immunofluorescent analysis of rat olfactory bulb section stained with Anti-MeCP2 Antibody (1:2,000 | red) and co-stained with Anti-alpha Synuclein Antibody (1:1,000 | green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-MeCP2 Antibody specifically labels the nuclei of neuronal cells while the Anti-alpha Synuclein Antibody reveals a-synuclein protein concentrated in presynaptic regions.
Western blot analysis of tissue and cell lysates using Anti-MeCP2 Antibody (1:20,000 | green): [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein. The MeCP2 proteins of rat and human origin are known to migrate slightly differently on SDS-PAGE gels compared to that of mouse, and as a result bands appear at somewhat different apparent molecular weights
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