Rabbit polyclonal antibody to MDM2 (Phospho-Ser186 / Ser188)
Specificity
The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.
Applications
IF
Dilutions
Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Ser186 / Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.