Anti-MDM2 (phospho Ser166) Antibody (A94434)

Name: Anti-MDM2 (phospho Ser166) Antibody
Brand: Antibodies.com
SKU: A94434
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to MDM2 (phospho Ser166) for WB, IHC, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-MDM2 (phospho Ser166) Antibody

Description

Rabbit polyclonal antibody to MDM2 (phospho Ser166).

Specificity

This antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:5000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide derived from human MDM2 around the phosphorylation site of Ser166 (amino acids 132-181).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

55kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, RING-type E3 ubiquitin transferase Mdm2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunofluorescence - Anti-MDM2 (phospho Ser166) Antibody (A94434)

Immunofluorescence analysis of A549 cells using Anti-MDM2 (phospho Ser166) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Immunohistochemistry - Anti-MDM2 (phospho Ser166) Antibody (A94434)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-MDM2 (phospho Ser166) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-MDM2 (phospho Ser166) Antibody (A94434)

Western blot analysis of lysates from COS7 cells using Anti-MDM2 (phospho Ser166) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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