Western Blot - Anti-MDM2 Antibody [ARC60296] (A305526)
Western blot analysis of U2OS, using Anti-MDM2 Antibody [ARC60296] (A305526) at 1:2,000 dilution. U2OS cells were treated by Nutlin 3a(10µM ) at 37°C for 24 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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