MDM2 expression in Human Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-MDM2 Antibody (A82614) at 0.3µg/ml and detected by chemiluminescence.
MDM2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MDM2 and incubated with Anti-MDM2 Antibody (A82614). Control: Mock-transfected HEK293 cell lysate incubated with Anti-MDM2 Antibody (A82614) (first lane).
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