Western blot analysis of mouse, rat brain showing detection of ~40 kDa MDM2 protein using Anti-MDM2 Antibody (A305002) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse Brain. Lane 3: rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~40 kDa. Other Band(s): Isoform 2 or 3.
Immunohistochemistry analysis of human stomach cancer, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-MDM2 Antibody (A305002) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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