Anti-MDM2 Antibody (A283100)

$340

Rabbit polyclonal antibody to MDM2 for WB and ELISA.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-MDM2 Antibody
Description
Rabbit polyclonal antibody to MDM2.
Specificity
This antibody recognises mouse Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle.Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.
Applications
WB, ELISA
Dilutions
ELISA: 1:3,000 - 1:12,000, WB: 1:500 - 1:2,000
Reactivity
Mouse
Immunogen
Synthetic peptide corresponding to amino acids 177-195 of mouse Mdm2.
Sequence
HRKRRRSLSFDPSLGLCEL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
500 µg/ml
Molecular Weight
Approximately 90 kDa in mouse MEF cell lysates.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Mouse Mdm2 antibody detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.
Synonyms
Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, RING-type E3 ubiquitin transferase Mdm2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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