Western blot analysis of extracts of NIH/3T3 cells, using Anti-MDM2 Antibody (A12616) at 1:1,000 dilution. NIH/3T3 cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Publishing research using Anti-MDM2 Antibody (A12616)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-MDM2 Antibody (A12616)
![Western Blot - Anti-MDM2 Antibody [SMP14] (A249312) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249312_1.jpg?profile=product_alternative)
![Western Blot - Anti-MDM2 Antibody [SMP14] - BSA and Azide free (A252492) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252492_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-MDM2 (phospho Ser166) Antibody [ARC2909] (A309403) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309403_1.jpg?profile=product_alternative)
