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Anti-MCP1 Antibody (Biotin) - BSA and Azide free (A282353)

$645

Rabbit polyclonal antibody to MCP1 (Biotin) for WB and ELISA.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-MCP1 Antibody (Biotin) - BSA and Azide free
Description
Rabbit polyclonal antibody to MCP1 (Biotin).
Specificity
This antibody recognises human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2.The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. MCP-1 is linked with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.Rabbit anti Human MCP-1 antibody has been successfully used as a detection reagent in the development of a sensitive ELISA assay for the evaluation of MCP-1 expression in conjunction with Mouse anti Human MCP-1, clone 2.2-4A4-1A11 (MCA2486) and recombinant human MCP-1 (PHP061) as a calibration reagent.
Applications
WB, ELISA
Dilutions
ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human MCP-1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Biotin

Concentration
100 µg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human MCP-1 antibody recognizes human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2.The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. MCP-1 is linked with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.Rabbit anti Human MCP-1 antibody has been successfully used as a detection reagent in the development of a sensitive ELISA assay for the evaluation of MCP-1 expression in conjunction with Mouse anti Human MCP-1, clone 2.2-4A4-1A11 (MCA2486) and recombinant human MCP-1 (PHP061) as a calibration reagent.
Synonyms
C-C motif chemokine 2, CCL2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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