General Notes
Mouse anti Human MCP-1, clone 5D3-F7 recognizes Monocyte chemoattractant protein-1, otherwise known as CCL2 (C-C motif chemokine 2), a member of the intercrine beta (chemokine CC) family, which acts as a chemoattractant for monocytes, basophils, natural killer cells (NKs) and memory T cells, in both normal and neoplastic tissues (Peri et al. 1994).MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.