This antibody recognises human Mcm-5 (minichromosome maintenance protein 5), also known as DNA replication licensing factor MCM5 or P1-CDC46. Mcm5 is a nuclear protein of ~95kDa with an important role in the control of DNA replication. Immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.
Applications
WB, IHC-P
Dilutions
IHC-P 1: 1:50 for 20 µg, 1:25 for 0.1 mg - 1:1,000 for 20 µg, 1:100 for 0.1 mg
Reactivity
Human
Immunogen
Bacterially expressed human Mcm5.
Host
Mouse
Clonality
Monoclonal
Clone ID
CRCT5.1
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human Mcm5 antibody, clone CRCT5.1 recognizes human Mcm-5 (minichromosome maintenance protein 5), also known as DNA replication licensing factor MCM5 or P1-CDC46. Mcm5 is a nuclear protein of ~95kDa with an important role in the control of DNA replication (Snyder et al. 2005).Immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy (Murphy et al. 2004).
Synonyms
CDC46, CDC46 homolog, DNA replication licensing factor MCM5, P1-CDC46