This antibody recognises the red fluorescent mCherry protein, a monomeric engineered derivate of a fluorescent protein isolated from members of the Discosoma (mushroom coral) family. mCherry is a second generation fluorescent protein and by far the most popular red emitting fluorescent protein due to its high photostability and size (the other photostable red fluorescent protein tdTomato due to its tandem dimer nature is significantly larger than the 29 kDa mCherry protein). mCherry is optimally excited at a wavelength of 587nm and emits light at 610nm. Fluorescent proteins are used as tags in the research environment and are fused to the N- or C-terminus of proteins of interest. They are tools enabling the visualization of proteins, protein-protein interactions and are used in cell-sorting and FRET, FRAP and FLIM imaging experiments.
Applications
WB
Dilutions
WB: 10 µg/ml
Immunogen
Recombinant mCherry expressed in E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
Clone 1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
500 µg/ml
Molecular Weight
Approximately 27 kDa on recombinant mCherry.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 5% Glycerol, 0.1% BSA, 0.05mM EDTA, and 0.03% ProClin 300.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti mCherry antibody recognizes the red fluorescent mCherry protein, a monomeric engineered derivate of a fluorescent protein isolated from members of the Discosoma (mushroom coral) family. mCherry is a second generation fluorescent protein and by far the most popular red emitting fluorescent protein due to its high photostability and size (the other photostable red fluorescent protein tdTomato due to its tandem dimer nature is significantly larger than the 29 kDa mCherry protein). mCherry is optimally excited at a wavelength of 587nm and emits light at 610nm. Fluorescent proteins are used as tags in the research environment and are fused to the N- or C-terminus of proteins of interest. They are tools enabling the visualization of proteins, protein-protein interactions and are used in cell-sorting and FRET, FRAP and FLIM imaging experiments.
