Rabbit polyclonal antibody to MAPKAPK2 (Phospho-Thr334)
Specificity
The antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at threonine 334.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2
Western blot analysis of extracts from NIH/3T3 cells, using MAPKAPK2 (Phospho-Thr334) antibody #12103. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK2 (Phospho-Thr334) antibody #12103. The picture on the right is treated with the synthesized peptide.
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