Rabbit polyclonal antibody to MAP3K1 (Phospho-Thr1402)
Specificity
The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1400.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 1400(K-G-T(p)-G-A) derived from Human MAP3K1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1400) Antibody #11737. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1400) antibody #11737 (left)or the same antibody preincubated with blocking peptide (right).
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