Mixture of recombinant fragments corresponding to amino acids 377-1505 of human MAP2 Projection P1, human MAP2 Projection P2, and human MAP2 Projection P3.
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
280 kDa (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunofluorescent analysis of rat brain stem section stained with Anti-MAP2A + MAP2B Antibody (1:2,000 | red), and co-stained with Anti-MBP Antibody (1:5,000 | green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The Anti-MAP2A + MAP2B Antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the Anti-MBP Antibody stains myelin sheath around axons.
Western blot analysis of whole brain lysates using Anti-MAP2A + MAP2B Antibody (1:5,000 | green): [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.
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