Anti-MAP2 Antibody [5H11] (A85296)

Immunofluorescent analysis of a rat cerebellum section stained with Anti-MAP2 Antibody [5H11] (A85296) at a dilution of 1:5,000 (green), and costained with Anti-alpha Internexin Antibody () at a dilution of 1:2,000 (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies. The Anti-MAP2 Antibody [5H11] (A85296) antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, particularly in granular cell and molecular layers of the cerebellum, while the a-internexin antibody selectively stains axons and dendrites of neuronal cells.

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat cerebellum section with Anti-MAP2 Antibody [5H11] (A85296) at a dilution of 1:5,000 detected in DAB (brown) following the ImmPress method strongly labels neurons, neuronal projections, and synapses. Note: this antibody performs well in testing with Anti-MAP2 Antibody [5H11] (A85296) is the recommended clone for MAP2 immunostaining.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human brain cortex section with Anti-MAP2 Antibody [5H11] (A85296) at a dilution of 1:5,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-MAP2 Antibody [5H11] (A85296) strongly labels neurons, neuronal projections, and synapses. This antibody performs well in testing with 4% PFA fixed or standard NBF fixed rat and human tissue but does not stain long term NBF fixed material effectively. Due to its strong staining profile and ability to stain human tissue, Anti-MAP2 Antibody [5H11] (A85296) is the recommended clone for MAP2 immunostaining.