Anti-MAP2 Antibody [4H5] (A85297)

Mixed neuron and glia cultures stained with Anti-MAP2 Antibody (green) and Anti-NF-H Antibody (A85336 | red) and DNA (blue). Anti-MAP2 Antibody reveals strong cytoplasmic staining for dendrites and perikarya, which does not overlap with the Anti-NF-H Antibody which primarily binds to axons.

Western blot of rat brain extract probed with Anti-MAP2 Antibody. Note that the strong single band running at about 280 kDa corresponds to MAP2.

Immunofluorescent analysis of a rat hippocampus section stained with Anti-MAP2 Antibody [4H5] (A85297), at a dilution of 1:2,000, in green, and co-stained with Anti-Fox3 Antibody (A85403), at a dilution of 1:2,000 in red. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-MAP2 Antibody [4H5] (A85297) labels MAP2 protein in the perikarya and dendrites of most neurons while the Anti-Fox3 Antibody (A85403) antibody selectively stains nuclei and proximal soma of neuronal cells.

Western blot analysis of tissue and cell lysates using Anti-MAP2 Antibody [4H5] (A85297), at a dilution of 1:10,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.