Anti-Leptin Receptor Antibody [ARC0454] (A305700)

Name: Anti-Leptin Receptor Antibody [ARC0454]
Brand: Antibodies.com
SKU: A305700
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0454] antibody to Leptin Receptor for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Leptin Receptor Antibody [ARC0454]

Description

Rabbit monoclonal [ARC0454] antibody to Leptin Receptor.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Leptin Receptor (P48357).

Sequence

EEQGLINSSVTKCFSSKNSPLKDSFSNSSWEIEAQAFFILSDQHPNIISPHLTFSEGLDELLKLEGNFPEENNDKKSIYYLGVTSIKKRESGVLLTDKSRV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0454

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD295, DB, HuB219, LEP-R, LEPR, OB receptor, OB-R, OBR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Leptin Receptor Antibody [ARC0454] (A305700)

Western blot analysis of extracts of various cell lines, using Anti-Leptin Receptor Antibody [ARC0454] (A305700) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Immunohistochemistry - Anti-Leptin Receptor Antibody [ARC0454] (A305700)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Leptin Receptor Antibody [ARC0454] (A305700) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Leptin Receptor Antibody [ARC0454] (A305700)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Leptin Receptor Antibody [ARC0454] (A305700) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Leptin Receptor Antibody [ARC0454] (A305700)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Leptin Receptor Antibody [ARC0454] (A305700) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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