Lemzoparlimab Biosimilar - Anti-CD47 Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to CD47.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG4
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) which can bind Anti-CD47 Humanized Antibody [Lemzoparlimab Biosimilar] - Azide free (A318886) in a linear range of 3.2-400 µg/ml.
Jurkat cell line were surface stained with Anti-CD47 Humanized Antibody [Lemzoparlimab Biosimilar] - Azide free (A318886) (blue histogram) and isotype control (red histogram) at 1 µg/ml followed by Goat Anti-Human IgG Antibody (Alexa 488).
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