LDHA expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-LDHA Antibody (A329561) at a dilution of 1:1000 and detected with chemiluminescence.
LDHA expression in lysates from wild type (WT) and LDHA knockdown (KD) 293T cells analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-LDHA Antibody (A329561) at a dilution of 1:1000 and detected with chemiluminescence.
LDHA expression in mouse kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-LDHA Antibody (A329561) at a dilution of 1:100.
LDHA expression in L929 cells analyzed by immunofluorescence. Staining was performed with Anti-LDHA Antibody (A329561) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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