Anti-LC3B Antibody (A80400)

Name: Anti-LC3B Antibody
Brand: Antibodies.com
SKU: A80400
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to LC3B for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Autophagosome Marker

Specifications

Name

Anti-LC3B Antibody

Description

Rabbit polyclonal antibody to LC3B.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100, IP: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-50 of human LC3B (NP_073729.1).

Sequence

MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

20 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-LC3B Antibody (A80400)

Western blot analysis of extracts of 293 cells, using Anti-LC3B Antibody (A80400). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Western Blot - Anti-LC3B Antibody (A80400)

Western blot analysis of extracts of various cell lines, using Anti-LC3B Antibody (A80400) at 1:1,000 dilution. Hela cells were treated by Chloroquine (50 µM) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-LC3B Antibody (A80400)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-LC3B Antibody (A80400) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-LC3B Antibody (A80400)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-LC3B Antibody (A80400) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.