Recombinant humanized monoclonal antibody to GPCR GPRC5D.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
G-protein coupled receptor family C group 5 member D
ELISA plates were pre-coated with Recombinant Human GPCR GPRC5D Protein (Fc Tag) (A318179) at 2 µg/ml (100 µl/well) which can bind LBL-034 (without CD3) Biosimilar - Anti-GPCR GPRC5D Antibody - BSA and Azide free (A324677) in a linear range of 3.20-400 ng/ml.
Flow cytometry analysis of 1µg/ml of LBL-034 (without CD3) Biosimilar - Anti-GPCR GPRC5D Antibody - BSA and Azide free (A324677) on Expi293 cells transfected with Human GPRC5D protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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