Anti-LAMP2 Antibody [M3/84] (A281000)

Anti-LAMP2 Antibody [M3/84]

Rat monoclonal [M3/84] antibody to LAMP2.

This antibody recognises mouse CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. CD107b is involved in aspects of leucocyte adhesion. The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilization procedure prior to staining.

WB, IHC-P, IP, Flow Cytometry, IHC-Fr

FC 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Mouse

Glycoproteins purified from mouse peritoneal macrophage membranes.

Rat

Monoclonal

M3/84

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD107b antibody, clone M3/84 recognizes murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between ~92-120 kDa. CD107b is involved in aspects of leucocyte adhesion (Kannan et al. 1996).The expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilization procedure prior to staining.

CD107 antigen-like family member B, CD107b, LAMP-2, LGP-96, Lysosome-associated membrane glycoprotein 2