Western Blot - Anti-LAMP2 Antibody [GL2A7] (A304947)
Western blot analysis of Human, mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Anti-LAMP2 Antibody [GL2A7] (A304947) at 1:500 for 1 hour at room temperature. Lane 1: MW ladder. Lane 2: human HEK293 lysate (20 µg). Lane 3: mouse 3T3NIH lysate (10 µg). Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was HRP Goat Anti-Rat at 1:100 for 1 hour at room temperature. Color Development: TMB solution for 5 minutes at room temperature. Predicted/Observed Size: ~100-110 kDa.
Immunocytochemistry/Immunofluorescence analysis of rabbit corneal endothelial cell (CEC), using Anti-LAMP2 Antibody [GL2A7] (A304947), at 1:1,000. The secondary antibody used was FITC Goat Anti-Rat (green).
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