Anti-LAMP2 Antibody [AC17] (A279631)

Anti-LAMP2 Antibody [AC17]

Mouse monoclonal [AC17] antibody to LAMP2.

This antibody recognises canine CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2. Immunofluorescence staining of MDCK cells with mouse anti dog CD107b, clone AC17 demonstrates staining patterns consistent with localization to lysozomes. This is supported by coincident staining of an exogenous lysozomal glycoprotein, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24. Mouse anti Dog CD107b antibody, clone AC17 immunoprecipitates a protein of ~95 kDa in MDCK cells which, following Endo F digestion to remove N-linked oligosaccharides, yields a core protein product of 40 kDa, indicating the heavily glycosylated nature of CD107b. The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins. While most (97% ) CD107b resides in the lysozomal environment in adherent MDCK cells in vitro, a small percentage is associated with the cell membrane. CD107b has been shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b. Transfection of a mink type II lung epithelial cell line with beta1-6-N-acetylglucosaminyl transferase V demonstrates the formation of large lysozomal vacuoles, termed multilamellar bodies (MLBs), having a very distinct phenotype with expression of CD107b, as indicated by immunofluorescent staining with clone AC17. These MLBs require lysozomal degradation via an autophagic pathway for their formation and may have implications for lysozomal storage diseases. CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway. Mouse anti Dog CD107b antibody, clone AC17 is suitable for use in electron microscopy.

WB, IP, Flow Cytometry, IF

FC 1: 1:50 - 1:200, WB: 1:100 - 1:500, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Canine, Mink, Human

This antibody does not cross react with Mouse or Rat

MDCK (Madin-Darby Canine Kidney) cells.

Mouse

Monoclonal

AC17

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Dog CD107b antibody, clone AC17 recognizes canine CD107b, otherwise known as lysosome-associated membrane protein 2 or LAMP-2. Immunofluorescence staining of MDCK cells with mouse anti dog CD107b, clone AC17 demonstrates staining patterns consistent with localization to lysozomes. This is supported by coincident staining of an exogenous lysozomal glycoprotein, avian LEP100 transfected into MDCK cells and detected using the anti LEP100 antibody clone CV24 (Nabi et al.1991).Mouse anti Dog CD107b antibody, clone AC17 immunoprecipitates a protein of ~95 kDa in MDCK cells which, following Endo F digestion to remove N-linked oligosaccharides, yields a core protein product of 40 kDa, indicating the heavily glycosylated nature of CD107b. The molecular weight of canine CD107b is typical of many lysozome-associated membrane proteins. While most (97%) CD107b resides in the lysozomal environment in adherent MDCK cells in vitro, a small percentage is associated with the cell membrane (Nabi et al.1991).CD107b has been shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b (Nabi et al.1993). Transfection of a mink type II lung epithelial cell line with beta1-6-N-acetylglucosaminyl transferase V demonstrates the formation of large lysozomal vacuoles, termed multilamellar bodies (MLBs), having a very distinct phenotype with expression of CD107b, as indicated by immunofluorescent staining with clone AC17. These MLBs require lysozomal degradation via an autophagic pathway for their formation and may have implications for lysozomal storage diseases (Hariri et al.2000). CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway. Mouse anti Dog CD107b antibody, clone AC17 is suitable for use in electron microscopy (Nabi et al.1991).

CD107 antigen-like family member B, CD107b, LAMP-2, LGP-96, Lysosome-associated membrane glycoprotein 2