Western blot analysis of extracts of various cell lines, using Anti-Lamin B1 Antibody (A92627) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Lamin B1 Antibody (A92627) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Lamin B1 Antibody (A92627) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Anti-Lamin B1 Antibody (A92627) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Confocal immunofluorescence analysis of HeLa cells using Anti-Lamin B1 Antibody (A92627) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
