Lactoferrin expression in Peripheral Blood Mononucleocyte lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Lactoferrin Antibody (A84604) at 0.05µg/ml and detected by chemiluminescence.
Lactoferrin expression in Human Blood Vessel analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Lactoferrin Antibody (A84604) at 2µg/ml and revealed with alkaline phosphatase (AP).
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