Gel: 10%+12%+15% SDS-PAGE Lane1: Human lymphoma tissue lysate Lane2: Human ovarian cancer tissue lysate Lane3: Human colon cancer tissue lysate Lysates: 40 µg per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds
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