Anti-KLF4 Antibody [ARC0721] (A308102)

Name: Anti-KLF4 Antibody [ARC0721]
Brand: Antibodies.com
SKU: A308102
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC0721] antibody to KLF4 for WB, IHC, ICC/IF and IP.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

iPSCs Marker

Specifications

Name

Anti-KLF4 Antibody [ARC0721]

Description

Rabbit monoclonal [ARC0721] antibody to KLF4.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474).

Sequence

PAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0721

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epithelial zinc finger protein EZF, EZF, GKLF, Gut-enriched krueppel-like factor, Krueppel-like factor 4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

Enlarge Image

Western Blot - Anti-KLF4 Antibody [ARC0721] (A308102)

Western blot analysis of extracts of various cell lines, using Anti-KLF4 Antibody [ARC0721] (A308102) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Western Blot - Anti-KLF4 Antibody [ARC0721] (A308102)

Western blot analysis of extracts of Mouse lung, using Anti-KLF4 Antibody [ARC0721] (A308102) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Enlarge Image

Immunohistochemistry - Anti-KLF4 Antibody [ARC0721] (A308102)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-KLF4 Antibody [ARC0721] (A308102) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-KLF4 Antibody [ARC0721] (A308102)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-KLF4 Antibody [ARC0721] (A308102) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.