This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Bovine, Human
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428.
Host
Mouse
Clonality
Monoclonal
Clone ID
Ki-67
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67, Proliferation marker protein Ki-67
Flow cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA. Surface staining by Anti-CD25 Antibody [MEM-181] (APC) was followed by permeabilization and nuclear staining by Anti-Ki-67 Antibody [Ki-67] (PE).
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