Anti-Ki67 Antibody (A270579)

Immunofluorescent analysis of mouse NIH/3T3 cells stained with Anti-Ki67 Antibody (A270579) at a dilution of 1:1,000, red. Nuclei were stained with Hoechst (blue). The Anti-Ki67 Antibody (A270579) stains Ki-67 protein predominantly expressed in nucleoli of cells in interphase, while cells in the quiescent G0 state do not express Ki-67 protein. Dividing cells express Ki67 surrounding condensed chromosomes.

Immunohistochemistry analysis of a 4%PFA fixed paraffin embedded rat testes section with Anti-Ki67 Antibody (A270579) at a dilution of 1:2,000. Anti-Ki67 Antibody (A270579) strongly labels the nuclei of spermatogenic cells. Note: this antibody was not tested for staining on NBF fixed tissues. Both rat and mouse tissues stain effectively with Anti-Ki67 Antibody (A270579).

Western blot analysis of cytosol and nuclear fractions from different cell line lysates using Anti-Ki67 Antibody (A270579) at a dilution of 1:1,000 (green). The lanes contain: [Lane 1] protein standard (red), [Lane 2] extracts of HeLa cells cytosol, [Lane 3] HeLa cells nuclear fraction, [Lane 4] HEK293 cytosol, [Lane 5] HEK293 nuclear fraction, [Lane 6] NIH/3T3 cytosol, [Lane 7] NIH/3T3 nuclear fraction, [Lane 8] C6 cytosol, and [Lane 9] C6 nuclear fraction. Strong bands above 250 kDa mark correspond to two major isoforms of Ki-67 protein expressed in rat NIH/3T3 and glioma cell line C6. Smaller fragments of these isoforms are also detected on the blot in all cell lines.