Anti-Ki67 Antibody (A104340)

Immunofluorescent analysis of HeLa cells stained with Anti-Ki67 Antibody (1:2,000 | red), and Anti-beta Tubulin Antibody (1:5,000 | green). The blue is DAPI staining of nuclear DNA. The Anti-Ki67 Antibody detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state. The Anti-beta Tubulin Antibody produces strong staining of cytoplasmic microtubules

Western blot analysis of equal amounts of cell lysates probed with Anti-Ki67 Antibody (1:5,000 | green): [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67 is a rather unstable short half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.