Anti-Ki67 Antibody (A104340)

Immunofluorescent analysis of HeLa cells stained with Anti-Ki67 Antibody (A104340), at a dilution of 1:2,000, in red, and Anti-beta Tubulin Antibody [1B12] (A85428), at a dilution of 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Anti-Ki67 Antibody (A104340) detects Ki67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67 forms a coat around condensed chromosomes. Ki67 is not detected in cells in the quiescent G0 state. Anti-beta Tubulin Antibody [1B12] (A85428) produces strong staining of cytoplasmic microtubules

Western blot analysis of equal amounts of cell lysates probed with Anti-Ki67 Antibody (A104340), at a dilution of 1:5,000, in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rapidly dividing HeLa cell cultures, and [Lane 3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67 is a rather unstable short half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.

Immunohistochemistry analysis of a formalin fixed paraffin embedded murine small intestine cross section with Anti-Ki67 Antibody (A104340) at a dilution of 1:10,000 detected in DAB (brown) following the ABC method. Counterstained with Hematoxylin (blue). Anti-Ki67 Antibody (A104340) specifically detects the nuclei of proliferating intestinal cells. Mouse select image at left for larger view.