Anti-Ki67 Antibody (A104333)

Immunofluorescent analysis of HeLa cells stained with Anti-Ki67 Antibody (A104333), at a dilution of 1:5,000, in red, and Anti-Fibrillarin Antibody [38F3] (A85370), at a dilution of 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by Anti-Fibrillarin Antibody [38F3] (A85370). In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but Fibrillarin positive.

High magnification confocal image of adult mouse hippocampus dentate region stained with Anti-Ki67 Antibody (A104333) at a dilution of 1:2,000 (red) and Anti-NeuN Antibody [1B7] (A85405) at a dilution of 1:2,000 (green). Nuclei were stained with Hoechst (blue). Top left is DNA, top right FOX3/NeuN, bottom left Ki67 and bottom right all three merged. Dividing cells are very rare in adult animals, but one can be seen in the center of the image. Chromosomes can be seen in blue and their Ki67 coating can be seen (red). The dividing cell is FOX3/NeuN negative and so is presumably a glial cell.

Mouse NIH-3T3 cells stained with Anti-Ki67 Antibody (A104333) at a dilution of 1:5,000 (red) and Anti-beta Tubulin Antibody [1B12] at a dilution of 1:2,000 (green). The Ki67 strongly stains the nuclei of dividing cells, but not quiescent cells. Nuclei were stained with Hoechst (blue).

Immunohistochemistry analysis of formalin fixed paraffin embedded human breast tissue including normal and cancer cells. Cancer cells divide rapidly and heavily express Ki67 and so stain strongly with the Anti-Ki67 Antibody (A104333).