Recombinant construct containing the N-terminal 300 amino acids of human Ki67 isotype 1, expressed in and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
6G3
Isotype
IgG1
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
345 kDa, 395 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
Please note, Ki67 proteins are very unstable and only expressed in large amounts in situations where many cells are dividing. As a result of the very short half life of Ki67 there are usually numerous fragments visible on western blots running below the major 395 kDa and 345 kDa bands.
Synonyms
Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67, Proliferation marker protein Ki-67
Immunofluorescent analysis of HeLa cells stained with Anti-Ki67 Antibody (1:2,000 | red). The blue is DAPI staining of nuclear DNA. The Anti-Ki67 Antibody stains Ki67 protein predominantly expressed in the nucleoli of rapidly dividing cells, while nearby quiescent cells express little or no Ki67. The Ki67 protein also coats the surface of condensed chromosomes as seen at the middle left of the image.
Western blot analysis of equal amounts of cell lysates using Anti-Ki67 Antibody (1:2,000 | green): [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345 and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.
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