Anti-Ki67 Antibody [5F86] (A270555)

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Anti-Ki67 Antibody [5F86] (A270555) at a dilution of 1:5,000 (red) and Anti-Fibrillarin Antibody [38F3] (A85370) at a dilution of 1:2,000, (green). Nuclei were stained with DAPI (blue). The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.

Immunohistochemistry analysis of a 4%.PFA fixed paraffin embedded rat testes section with Anti-Ki67 Antibody [5F86] (A270555) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). In testes, the Anti-Ki67 Antibody [5F86] (A270555) antibody strongly labels the nuclei of spermatogenic cells. This antibody was not tested for staining NBF fixed tissues. Both rat and mouse tissues stain effectively with Anti-Ki67 Antibody [5F86] (A270555).

Western blot analysis of cell lysates from various cell lines using Anti-Ki67 Antibody [5F86] (A270555) at a dilution of 1:1,000, (green). The lanes contain: [Lane 1] protein standard (red), [Lane 2] extracts of mouse NIH/3T3, [Lane 3] rat glioma C6 cells. Strong bands above 250kDa correspond to two major isoforms of Ki67 protein expressed in the rodent cell lines and smaller fragments of these isoforms are also detected on the blot. We also tested extracts of [Lane 4] human cell lines, [Lane 5] HEK293, [Lane 6] HeLa, [Lane 6] SH-SY5Y cells, [Lane 7]. This antibody binds an epitope not conserved in human cells and so fails to stain these extracts.