Anti-Ki67 Antibody [5F86] (A270555)

Anti-Ki67 Antibody [5F86]

Mouse monoclonal [5F86] antibody to Ki67.

WB, ICC/IF, IHC

WB: 1:5,000-1:10,000, IF: 1:2,000-1:5,000, IHC: 1:1,000

Rat, Mouse

This antibody is not recommended for use on human tissues.

Recombinant fragment corresponding to amino acids 956-1,322 of mouse Ki67, a region corresponding to 2nd, 3rd, and 4th Ki67 type repeats, expressed in and purified from E. coli.

Mouse

Monoclonal

5F86

IgG1

1 mg/ml

345 kDa, 395 kDa

Liquid

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Please note, Ki67 proteins are very unstable and only expressed in large amounts in situations where many cells are dividing. As a result of the very short half life of Ki67 there are usually numerous fragments visible on western blots running below the major 395 kDa and 345 kDa bands.

Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67, Proliferation marker protein Ki-67