Anti-Keap1 Antibody (A13680)

Name: Anti-Keap1 Antibody
Brand: Antibodies.com
SKU: A13680
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Keap1 for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Keap1 Antibody

Description

Rabbit polyclonal antibody to Keap1.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

MCF7, HepG2, 293T, Mouse ovary, Rat brain.

Dilutions

WB: 1:100-1:500, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 320-510 of human Keap1.

Sequence

RAPKVGRLIYTAGGYFRQSLSYLEAYNPSDGTWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCAPMSVPRNRIGVGVIDGHIYAVGGSHGCIHHNSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNRLNSAECYYPERNEWRMITAMNTIRSGA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60-64 kDa

Predicted MW

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cytosolic inhibitor of Nrf2, INRF2, Kelch-like ECH-associated protein 1, Kelch-like protein 19, KIAA0132, KLHL19

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Keap1 Antibody (A13680)

Keap1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Keap1 Antibody (A13680) at a dilution of 1:500 and detected with chemiluminescence.

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Immunohistochemistry - Anti-Keap1 Antibody (A13680)

Keap1 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50.

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Immunohistochemistry - Anti-Keap1 Antibody (A13680)

Keap1 expression in human esophageal cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50.

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Immunofluorescence - Anti-Keap1 Antibody (A13680)

Keap1 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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