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Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610)

Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.com Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.com Chromatin Immunoprecipitation - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to KDM5A/Jarid1A/RBBP2 for WB, IP and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-KDM5A/Jarid1A/RBBP2 Antibody
Description
Rabbit polyclonal antibody to KDM5A/Jarid1A/RBBP2.
Applications
WB, IP, ChIP
Dilutions
WB: 1:500-1:2,000, IP: 1:100-1:500, ChIP: 1:100-1:500
Reactivity
Human, Rat
Immunogen
Recombinant protein of human KDM5A.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
192 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610)

Western blot analysis of extracts of various cell lines, using Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610). This Western blot was performed on the immunoprecipitate using Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) at a dilution of 1:500.
Chromatin Immunoprecipitation - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) - Antibodies.comEnlarge Image

Chromatin Immunoprecipitation - Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HCT116 cells, using Anti-KDM5A/Jarid1A/RBBP2 Antibody (A15610) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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