Anti-KDM1A Antibody (A13332)

Name: Anti-KDM1A Antibody
Brand: Antibodies.com
SKU: A13332

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This product is discontinued

Anti-KDM1A Antibody (A13332) has been discontinued and is no longer available.

View all KDM1A Antibodies.

Specifications

Name

Anti-KDM1A Antibody

Description

Rabbit polyclonal antibody to KDM1A.

Applications

WB, IF, IP

Dilutions

WB: 1:500-1:2000, IF: 1:50-1:100, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1A (NP_055828.2).

Sequence

LSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

96 - 117kDa (Observed MW)

Formulation

Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AOF2, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-KDM1A Antibody (A13332)

Western blot analysis of extracts of various cell lines, using Anti-KDM1A Antibody (A1156) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020).

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Western Blot - Anti-KDM1A Antibody (A13332)

Western blot analysis of extracts from normal (control) and KDM1A knockout (KO) HeLa cells, using Anti-KDM1A Antibody (A1156) at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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Immunofluorescence - Anti-KDM1A Antibody (A13332)

Immunofluorescence analysis of A549 cells using Anti-KDM1A Antibody (A1156).

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Immunoprecipitation - Anti-KDM1A Antibody (A13332)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg Anti-KDM1A Antibody (A1156). Western blot was performed from the immunoprecipitate using Anti-KDM1A Antibody (A1156) at a dilition of 1:500.