Western Blot - Anti-KDM1 / LSD1 Antibody (A306723)
Western blot analysis of Mouse brain, using Anti-KDM1 / LSD1 Antibody (A306723) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-KDM1 / LSD1 Antibody (A306723) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-KDM1 / LSD1 Antibody (A306723) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-KDM1 / LSD1 Antibody (A306723) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal immunofluorescence analysis of Hela cells using Anti-KDM1 / LSD1 Antibody (A306723) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).