Anti-KDEL Antibody (A305067)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-KDEL Antibody (A305067), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of human Cell line lysates showing detection of KDEL protein using Anti-KDEL Antibody (A305067) at 1:1000, 1:500.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-KDEL Antibody (A305067), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-KDEL Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.