Western Blot - Anti-IRS1 Antibody [ARC0486] (A308798)
Western blot analysis of extracts of HepG2 cells, using Anti-IRS1 Antibody [ARC0486] (A308798) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
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