Western blot analysis of extracts of various cell lines, using Anti-IRF3 Antibody (A11118) at 1:3000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using Anti-IRF3 Antibody (A11118) at 1:3000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using Anti-IRF3 Antibody (A11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
![Flow Cytometry - Anti-IRF3 Antibody [PCRP-IRF3-6C8] (A249050) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249050_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-IRF3 Antibody [PCRP-IRF3-6C8] - BSA and Azide free (A252230) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252230_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-IRF3 Antibody [PCRP-IRF3-1E6] (A249049) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249049_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-IRF3 Antibody [PCRP-IRF3-1E6] - BSA and Azide free (A252229) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252229_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-IRF3 Antibody [PCRP-IRF3-3B2] (A277870) - Antibodies.com](https://cdn.antibodies.com/image/catalog/277/A277870_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-IRF3 Antibody [PCRP-IRF3-3B2] - BSA and Azide free (A278458) - Antibodies.com](https://cdn.antibodies.com/image/catalog/278/A278458_1.jpg?profile=product_alternative)
