Anti-IRF3 Antibody (A80489)

Name: Anti-IRF3 Antibody
Brand: Antibodies.com
SKU: A80489
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to IRF3 for WB, IHC-P, ICC/IF, IP, ChIP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-IRF3 Antibody

Description

Rabbit polyclonal antibody to IRF3.

Applications

WB, IHC-P, ICC/IF, IP, ChIP, ELISA

Positive Control

293T.

Dilutions

WB: 1:1,000-1:5,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration), ChIP: 5µg antibody for 10µg-15µg of Chromatin

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 100-200 of human IRF3.

Sequence

PHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

47 kDa

Predicted MW

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Interferon regulatory factor 3, IRF-3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-IRF3 Antibody (A80489)

IRF3 expression in 293T cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-IRF3 Antibody (A80489) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-IRF3 Antibody (A80489)

IRF3 expression in human lung cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-IRF3 Antibody (A80489) at a dilution of 1:100.

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Immunohistochemistry - Anti-IRF3 Antibody (A80489)

IRF3 expression in human esophageal cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-IRF3 Antibody (A80489) at a dilution of 1:100.

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Immunofluorescence - Anti-IRF3 Antibody (A80489)

IRF3 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-IRF3 Antibody (A80489) at a dilution of 1:100 followed by ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).